Filamentous fungi or mold live everywhere in Japan . Filamentous fungi are hated because they damage foodstuff, such as bread, rice cake and grow under the humid condition, making environment uncomfortable or nasty. On the contrary, filamentous fungi are recognized as important organisms because they have been used for fermentation industry to make Japanese sake, soy sauce in Japan and cheese in Europe .

  Moreover filamentous fungi also contributed to the development of medical technology since the discovery of antibiotic PenicillinE in 1929 as a resource for screening and/or a tool for mass production of physiological compounds such as antibiotics, anticancer drug because they show high productivity of secondary metabolites.

   As a result of advanced genome analysis by genetic technology, it was revealed that filamentous fungi have ability to produce as many as 50 different compounds and are promising resources for discovery of novel compounds. Nowadays new bioactive compounds are highly demanded because of the emergence of novel pathogens, multidrug resistance bacteria and novel diseases. Making the most of the bioresource will bring about huge benefits to the life of human being.

    We consider filamentous fungi as bioresources having infinite possibility and aim to contribute to the society by applying them efficiently.

  • Search for novel bioactive compounds produced by filamentous fungi.
  • Identification of biosynthetic genes for bioactive compounds and analysis of the regulatory mechanism for their expression.
  • Construction of heterologous production system of fungal bioactive compounds
  • Synthesis of novel compounds by genetic techniques.
  • Molecular breeding by genetic technology.
2011, Nov.08th updated    
*Keiichi ISHIDO PhD student
*Ratklao SIRIWACH PhD student Thai
*Erika Takahashi Master student
*Noriko Miwa Master student  
*Nguyen Thi Minh Viet Master student Vietnam
*Nguyen Nhu Ha Vy Master student Vietnam
*Keisuke Yamanaka Undergraduate
*Mariko Yoshioka Undergraduate
Back to Top
Publication /// 2011 Nov. 08th updated
Sakai K, Kinoshita H, Nihira T.
Heterologous expression system in Aspergillus oryzae for fungal biosynthetic gene clusters of secondary metabolites.
Appl Microbiol Biotechnol. 2011 [PubMed]
Siriwach R, Kinoshita H, Kitani S, Igarashi Y, Pansuksan K, Panbangred W, Nihira T.
Xylaropyrone, a new γ-pyrone from the endophytic fungus Xylaria feejeensis MU18.
Journal of Antibiotics 2011 Feb;64(2):217-9. [PubMed]
Singkaravanit S, Kinoshita H, Ihara F, Nihira T.
Cloning and functional analysis of the second geranylgeranyl diphosphate synthase gene influencing helvolic acid biosynthesis in Metarhizium anisopliae.
Appl Microbiol Biotechnol. 2010 Apr 15. 87(3):1077-88. [PubMed]
Putri SP, Kinoshita H, Ihara F, Igarashi Y, Nihira T.
Ophiosetin, a new tetramic acid derivative from the mycopathogenic fungus Elaphocordyceps ophioglossoides.
J Antibiot (Tokyo). 2010 Apr;63(4):195-8. [PubMed]
Singkaravanit S., Kinoshita H., Ihara F., Nihira T.
Geranylgeranyl diphosphate synthase genes in entomopathogenic fungi. Appl Microbiol Biotechnol.
Appl Microbiol Biotechnol. 2010 Feb;85(5):1463-72. [PubMed]
Sakai K., Kinoshita H., Nihira T.
Identification of mokB involved in monacolin K biosynthesis in Monascus pilosus.
Biotechnol Lett. 2009 Dec:31(12):1911-6 [PubMed]
Sastia P. Putri, Kinoshita H., Ihara F., Igarashi Y., Nihira T.
Farinomalein, a maleimide-bearing compound from the entomopathogenic fungus Paecilomyces farinosus
Journal of Natural Products 2009 Aug;72(8):1544-6.  [PubMed]
Baba S., Kinoshita H., Hosobuchi M., Nihira T.
MlcR, a zinc cluster activator protein, is able to bind to a single (A/T)CGG site of cognate asymmetric motifs in the ML-236B (compactin) biosynthetic gene cluster.
Mol Genet Genomics. 2009 Jun;281(6):627-34 [PubMed]
Sakai K., Kinoshita H., Shimizu T., and Nihira T.
Construction of a citrinin gene cluster expression system in heterologous host Aspergillus oryzae.
J Biosci Bioeng 2008 Nov;106(6):466-72 [PubMed]
Lee SY., Ihara F., Igarashi Y., Kinoshita H., and Nihira T.
Identification of novel derivative of helvolic acid from Metarhizium anisopliae grown in insect-component medium.
J Biosci Bioeng. 2008 May;105(5):476-80. [PubMed]
Azumi M., Ishidoh K., Kinoshita H., Nihira T., Ihara F., Fujita T., and Igarashi Y.
Aurovertins F-H from the entomopathogenic fungus Metarhizium anisopliae.
J Nat Prod. 2008 Feb;71(2):278-80 [PubMed]
Shimizu T, Kinoshita H, Nihira T.
Identification and in vivo functional analysis by gene disruption of ctnA, an activator gene involved in citrinin biosynthesis in Monascus purpureus
Appl Environ Microbiol 2007 Aug;73(16):5097-103 [PubMed]
Shimizu T, Kinoshita H, Nihira T.
Development of transformation system in Monascus purpureus using an autonomous replication vector with aureobasidin A resistance gene.
Biotechnol Lett. 2006 Jan;28(2):115-20. [PubMed]
Lee SY, Nakajima I, Ihara F, Kinoshita H, Nihira T.
Cultivation of entomopathogenic fungi for the search of antibacterial compounds. Mycopathologia. 2005 Nov;160(4):321-5. [PubMed]
Shimizu T, Kinoshita H, Ishihara S, Sakai K, Nagai S, Nihira T.
Polyketide synthase gene responsible for citrinin biosynthesis in Monascus purpureus.
Appl Environ Microbiol. 2005 Jul;71(7):3453-7.[PubMed]
Back to Top
Back to Top